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A Study of Biofilm Production in Staphyloccus aureus

Sarita Yadav 1, Madhu Sharma 2, Aparna Yadav 2, and Uma Chaudhary 2
1 Department of Microbiology BPS, GMC for Women, Khanpur Kalan, Sonepat, Haryana.
2 Department of Microbiology, Pt. B.D. Sharma PGIMS, Rohtak, Haryana, India.

Abstract—Aims and objectives: (1) To detect biofilm formation in clinical isolates of Staphylococcus aureus. (2) To know the antimicrobial resistance pattern of the isolates and correlation with biofilm production. Methodology: The present study was done to detect biofilm formation in 50 clinical isolates of Staphylococcus aureus from blood and indwelling devices. For detection of biofilm formation, the clinical isolates were screened by Modified Tissue Culture Plate method (MTCP) and Tube Method (TM).Antibiotic susceptibility of the isolates was determined by using Kirby- Bauer disc diffusion method. Results: Out of 50 isolates of Staphylococcus aureus , 46% were biofilm positive by MTCP and 38% by TM. The biofilm producers were multi drug resistant as compared to the non producers. Interpretation: The effective control for staphylococcal infections will require a concerted effort to develop therapeutic agents that target the biofilm phenotype.

Index Terms—S. aureus , Biofilms, Multidrug resistance, Antibiotics

Cite: Sarita Yadav, Madhu Sharma, Aparna Yadav, and Uma Chaudhary, "A Study of Biofilm Production in Staphyloccus aureus," International Journal of Pharma Medicine and Biological Sciences, Vol. 3, No. 2, pp. 61-63, April 2014.
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