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Copper Induced Oxidative Stress Modulate Activities of CA2+ - Dependent Enzymes in Neurospora crassa

Pavan K Manikonda and C Subramanyam
Department of Biochemistry, University College of Science, Osmania University, Hyderabad 500007, AP, India.
Abstract—Employing the well-studied model of copper induced oxidative stress in Ascomycete fungus, Neurospora crassa , the present investigation was conducted to ascertain the correlations between copper induced oxidative stress and Ca 2+ -homeostasis. Transient exposure of a wild strain of N. crassa to 0.5 mM copper lead to increased reactive oxygen species, lipid peroxidation, activities of antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase and decreased the ratio of reduced to oxidized glutathione (p \< 0.05). Presence of 5 mM H2 O2 in culture media enhanced the copper induced oxidative stress conditions as well as increased intracellular Ca 2+ levels that could be attenuated by antioxidant, 10 mM N-acetyl cysteine (NAC). Copper induced stress caused decrease in the activities of Ca 2+ -dependent protein kinase C (PKC) and calcineurin (p < 0.05). On the contrary, there was no change in calmodulin and calcineurin contents and the activity of Ca 2+ -calmodulin dependent protein kinase II in response to copper. Those changes induced by copper enhanced by H 2 O 2 and attenuated with NAC. In light of these observations it may suggest that oxidative stress is indeed associated with significant alterations of Ca 2+ -signaling enzymes involved in regulatory pathways in this lower eukaryote.

Index Terms—Calcineurin, Glutathione, Metallothionein, Protein kinase C, Reactive oxygen species

Cite: Pavan K Manikonda and C Subramanyam, "Copper Induced Oxidative Stress Modulate Activities of CA2+ - Dependent Enzymes in Neurospora crassa," International Journal of Pharma Medicine and Biological Sciences, Vol. 3, No. 2, pp. 64-76, April 2014.
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