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The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijpmbs@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
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Discrimination Colonies of Staphylococcus aureus and Salmonella enterica by Using Machine Learning

Manao Bunkum and Sarinporn Visitsattapongse
Department of Biomedical Engineering, School of Engineering, King Mongkut’s Institute of Technology Ladkrabang Bangkok, Thailand

Abstract—Discrimination of bacteria is necessary because bacteria are remarkably crucial as they can contaminate in many possible ways such as food, water, air or environment, and lead to pathogenesis. In recent times, the technique of discrimination and counting bacteria are not only have a large expenditure of time and funds, but the discrimination method also required an expert in field. According to above, this research introduces an algorithm for solve those problems by using Staphylococcus aureus and Salmonella enterica as a pilot study. The stab technique, to inoculated bacteria in Luria-Bertani agar (LB agar) plates, was used for providing a good detection. In addition, the image segmentation technique was invited to train the separate colony of each bacterium for machine learning algorithm. It took approximately 800 images of bacteria for training process. The algorithm had resulted in 98.75 % of accuracy in bacteria’s colony counted and 98.12% in discriminated between Staphylococcus aureus and Salmonella enterica from stab technique. The algorithm had resulted in 33.61 % of accuracy in bacteria’s colony counted and 41.73% in discriminated between Staphylococcus aureus and Salmonella enterica from spread technique

Index Terms—bacteria, Staphylococcus aureus, Salmonella enterica, webcam camera, machine learning algorithm

Cite: Manao Bunkum and Sarinporn Visitsattapongse, "Discrimination Colonies of Staphylococcus aureus and Salmonella enterica by Using Machine Learning," International Journal of Pharma Medicine and Biological Sciences, Vol. 10, No. 1, pp. 30-34, January 2021. doi: 10.18178/ijpmbs.10.1.30-34

Copyright © 2021 by the authors. This is an open access article distributed under the Creative Commons Attribution License (CC BY-NC-ND 4.0), which permits use, distribution and reproduction in any medium, provided that the article is properly cited, the use is non-commercial and no modifications or adaptations are made.
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