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Biosynthesis and Characterization of Silver Nanoparticles Produced by Bacillus licheniformis

Shaarika Sarangadharan and Sivakumar Nallusamy
Department of Biology, College of Science, Sultan Qaboos University, Muscat, Oman
Abstract—Silver nanoparticles were synthesized using Bacillus licheniformis isolated from Al Thwara hot spring, Oman. Both supernatant and biomass of B. licheniformis were tested for their ability to reduce silver nitrate solution to silver nanoparticles by adding 1 and 3mM silver nitrate solutions. Silver nanoparticle production was observed both in supernatant and biomass B. licheniformis. Biosynthesis of silver nanoparticles occurred in presence of light. The particles maintained a good stability over time course. Small sized silver nanoparticles were produced when using 1 mM silver nitrate solution. The particle size ranges from 3 to 130 nm in supernatant with 1mM silver nitrate and 45-170 nm in supernatant with 3mM silver nitrate. On the other hand, 3 to 130nm and 45-168 nm particle size have been observed in biomass with 1mM and 3mM silver nitrate respectively. The average size of silver nanoparticles was 66 nm for 1 and 3mM silver nitrate solution with supernatant and 110 and 107 nm for 1 and 3mM silver nitrate solution with biomass respectively. However, AgNPs with 35 and 38nm size had a high percent intensity when 1mM AgNO3 was used with supernatant and biomass.

Index Terms—silver nanoparticles, B. licheniformis, stability of silver nanoparticles, DLS, supernatant, biomass

Cite:Shaarika Sarangadharan and Sivakumar Nallusamy, "Biosynthesis and Characterization of Silver Nanoparticles Produced by Bacillus licheniformis," International Journal of Pharma Medicine and Biological Sciences, Vol. 4, No. 4, pp. 236-239, October 2015.

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