Abstract—A simple, sensitive, accurate and precise LC assay method was developed for the quantitative determination of Ziprasidone Hydrochloride (ZSH) in pharmaceutical dosage form. Chromatographic separation was achieved by use of Lichrospher RP-18 column (250 ¡Á 4.0 mm, 5 m), using 20 mM ammonium acetate (pH adjusted to 3.0 with orthophosphoric acid) and methanol (30:70 %v/v) as mobile phase at 25 o C. The described method was linear over a range of 1-500 g mL-1 for determination of ZSH (r= 0.9998). F-test and t-test at 95% confidence level were used to check the intermediate precision data obtained under different experimental setups; the calculated value was found to be less than critical value. The developed method was found to be simple, specific, robust, linear, precise, and accurate for the determination of ZSH in pharmaceutical formulations.