Abstract—The repair of surgical wounds and tissue defects remains a significant clinical challenge, often necessitating advanced wound management strategies. Inspired by the traditional use of Periplaneta americana and snake slough in wound healing, this study developed a novel bioadhesive by integrating their bioactive peptide extracts within a deep eutectic solvent framework. The three components form a homogeneous composite system through strong eutectic effects and self-assembly, solidifying at the affected area via a simple preheating-cooling method. The deep eutectic solvent serves multiple functions as a co-solvent, plasticizer, penetration enhancer, and antibacterial agent. The preparation process is rapid, mild, and scalable, utilizing green raw materials with no additional additives. The resulting bioadhesive exhibited a temperature-induced sol-gel transition, enabling convenient application and in situ solidification. Due to the presence of hydrogen bonding, electrostatic interactions, and van der Waals forces, the prepared bioadhesive exhibited excellent adhesive properties. In vitro release studies demonstrated a sustained polypeptide release profile. Stability assessment over 90 days at room temperature showed no significant changes in polypeptide content, appearance, or odor, owing to the stabilizing and antimicrobial properties of the deep eutectic solvent. This multifunctional bioadhesive, leveraging the synergistic therapeutic effects of its components, shows great potential for surgical repair and wound healing applications.
 
Keywords—deep eutectic solvent, bioadhesive, two animal peptides, multifunctional material, wound healing

Cite: Zidan Luo, Ruirui Li, and Shun Yao, "A Deep Eutectic Solvent Bioadhesive Based on a Mixture of Peptides from Periplaneta americana and Snake Slough," International Journal of Pharma Medicine and Biological Sciences, Vol. 15, No. 4, pp. 162-166, 2026.

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