Abstract—The study aimed to evaluate the phytochemicals, antioxidant and antibacterial activity of Terminalia catappa leaves ethanolic extract. The extract was evaluated by phytochemical qualitative reactions for plant metabolites. The test was performed for flavonoids, phenols/tannins, and terpenoids for three trials. The color intensity or the precipitate formation was used as responses to these tests. The free radical scavenging activity of the ethanolic extract was quantitatively assessed using the DPPH radical method adopted for spectrophotometry. The antibacterial screening of Talisay extract against Staphylococcus aureus used the Kirky-Bauer Disk Diffusion method. Results of phytochemical showed that there is the presence of phenols/tannins, flavonoids and terpenoids based on the qualitative testing conducted on the test samples. Results of percentage DPPH scavenging activity of the ascorbic acid revealed substantially higher antioxidant activity (34.33%±0.6597) when compared to Talisay extract (23.88%±2.9621). Results of the mean diameter zone of inhibition of Talisay showed positive antibacterial activity with a value of 13.3±1.1547. The findings clearly indicated that the plant extract used has potential antioxidant and antibacterial agents. Demonstration of phytochemicals, antioxidant and antibacterial activity of Terminalia catappa provides the scientific basis and may help to discover new chemical classes of antioxidant and antibacterial substances that could serve as selective agents against degenerative diseases. This investigation has opened up the possibility of the use of Talisay leaves extract in producing an antioxidant and antibacterial agents that function as an alternative to highly available antioxidant and antibacterial product in drug stores.
Cite: Kenneth Neil O. Orillaneda, Emmanuel S. Caracal, Ikn Dzaia Erika A. Del Campo, Alyssa Kyla T. Flores, Lorelie M. Malda, Coleen Eve B. Morales, and Chloe Jane D. Reniedo, "Phytochemical Screening, Antioxidant, and Antibacterial Property of Talisay (Terminalia catappa) Leaves Ethanolic Extract Against Staphylococcus aureus Used as Formulated Ointment," International Journal of Pharma Medicine and Biological Sciences, Vol. 11, No. 4, pp. 89-95, October 2022. doi: 10.18178/ijpmbs.11.4.89-95

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