Abstract—The objective was to conduct a comparative study of estrogenic activity of alcoholic extract of fruits of Solanum xanthocarpum with diethylstilbestrol in bilaterally ovariectomized young albino rats using uterine wet weight, uterine glycogen content and uterine histology as parameters of assessment. Materials and Methods: Bilaterally overiectomized albino rats were divided into five groups (n=7) receiving different treatments, consisting of vehicle (distilled water), ethanolic extract of fruits of Solanum xanthocarpum at three different doses (viz., 100, 200, 400 mg/kg body weight) and standard drug diethylstilbestrol (DES) at a dose of 2 mg/kg body weight. All drugs were administered orally daily for 7 days. Estrogenic activity was assessed by taking uterine wet weight, uterine glycogen content, and uterine histology as parameters of assessment. Results: The results proved the estrogenic activity of extracts for dose 200 & 400 mg/kg body weight by exhibiting the significant ( p\<0.05 & p\<0.01) result for various parameters like uterine wet weight, uterine glycogen content and uterine histology. But the dose 100 mg/kg of Solanum xanthocarpum was proved statistically insignificant in above mentioned parameters. Conclusion: Solanum xanthocarpum showed moderate estrogenic activity in a dose dependent manner compared to diethylstilbestrol.