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Effect of Different Trypsin Concentrations on SMCC-7721 Cell Adhesion

Jin Yan1,2, Liguo Tian1,2, Chenchen Xie1,2, Yan Liu1,2, Zhengcheng Lu1,2, and Zuobin Wang1,2,3
1.International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China
2.Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China
3.IRAC & JR3CN, University of Bedfordshire, Luton LU1 3JU, UK
Abstract—In this work, living human liver cancer cells (SMCC-7721) were exposed to the trypsin solution at the concentrations of 1.5 mg/ml, 2.0 mg/ml, 2.5 mg/ml, 3.0 mg/ml, 3.5 mg/ml and 4.0 mg/ml, respectively. After 2 min of dosing time, the physical and mechanical properties of the cells were detected by Atomic Force Microscope (AFM). With the increase of the trypsin concentration, the adhesion force between the cell and the probe gently decreased. The maximum lateral deflection voltage variation of the probe, which reflected the maximum external force that kept the cells attached to the substrate, was significantly reduced. This work indicates that we may change the cell morphology and regulate the mechanical properties of cells by controlling the concentration of trypsin solution to treat cells, which has important implications for bioengineering and cell manipulation.

Index Terms—atomic force microscope, cell adhesion, trypsin treatment, SMCC-7721 cell

Cite: Jin Yan, Liguo Tian, Chenchen Xie, Yan Liu, Zhengcheng Lu, and Zuobin Wang, "Effect of Different Trypsin Concentrations on SMCC-7721 Cell Adhesion," International Journal of Pharma Medicine and Biological Sciences, Vol. 10, No. 3, pp. 103-107, July 2021. doi: 10.18178/ijpmbs.10.3.103-107

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