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The journal receives submitted manuscripts via email only. Please submit your research paper in .doc or.pdf format to the submission email: ijpmbs@ejournal.net.
You’ll be given a paper number if your submission is successful. Your paper then will undergo peer review process, which may take approximately one and a half months under normal circumstances, three tops.
After blind peer review, you will receive the notification letter with the review result of your paper...
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The journal publishes full research papers.[Read More]
 

Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples

Mohd Akmal Azhar and Mimi Sakinah Abdul Munaim
Faculty of Engineering Technology, Universiti Malaysia Pahang, Kuantan, Malaysia
Abstract—Kefir drink is a product from the fermentation process of milk using symbiotic mixture of bacteria and yeast consortium. Saccharomyces and lactobacillus are the major genera found in the kefir drink. The present work involves the isolation and identification of potential probiotic yeast strains in the kefir drink samples from Malaysia. The molecular identification was done by PCR using ITS1 and ITS4 amplified regions. Nine different yeast strains were isolated, and the strains were successfully identified based on the sequence analysis. Saccharomyces and Kodamaea were found to be the major population in the kefir drink samples. Lastly, kefir milk is one of the excellent sources of probiotic yeast strains and could be used as a new yeast probiotic formulation or in food supplements. Moreover, the amplification of ITS region can be used as a useful method to identified yeast strains.

Index Terms—kefir, yeast, probiotics, molecular identification, PCR

Cite:Mohd Akmal Azhar and Mimi Sakinah Abdul Munaim, "Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples," International Journal of Pharma Medicine and Biological Sciences, Vol. 8, No. 4, pp. 128-131, October 2019. doi: 10.18178/ijpmbs.8.4.128-131

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