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Combination Formulated Herbals (Pimpinella alpina, Eurycoma longifolia, and Curcuma xanthorrhiza) Increases Number and Improves the Quality of Sperm in Male Rat

Ronny Lesmana1,4,5,6, Hanna Goenawan1,5, Aziiz M. Rosdianto1,4,5, Akbar Haqi2, Susianti5, and Unang Supratman3,5
1.Division of physiology, Department of Anatomy, Physiology, and Biology Cell, Faculty of Medicine, Universitas Padjadjaran, Indonesia
2.Resource Biotechnology, Department of Molecular Biology, Faculty of Resource Sciences and Technology, University of Malaysia Sarawak, Indonesia
3.Faculty of Mathematics and Natural Science , Departement of Chemistry, Universitas Padjadjaran, Indonesia
4.Veterinary Medicine Study Program, Faculty of Medicine, Universitas Padjadjaran, Indonesia
5.Central Laboratory, Universitas Padjadjaran, Indonesia
6.Center of Excellent in Higher Education For Pharmaceutical Care Innovation, Universitas Padjadjaran, Indonesia
Abstract—Number fertility cases increased significantly within the decade all over the world including Indonesia, therefore finding a new agent which can stimulate or improve fertility condition is very important. In the present study, we explore effect of combination formulated herbals (Pimpinella Alpina, Eurycoma longifolia, Curcuma xanthorrhoea) which is used traditionally in Indonesia as anti infertility agent. Twelve male wistar rats were divided into 2 groups control and treatment for 2 weeks. Rat was treated with formulated herbal with dose concentration is 0.009 g/rats via gavaging, then rats were sacrificed under ether anesthesia. Epididymis was excised and sperm was collected, stained using 2% for exploring changes in motility and viability of sperm under a light microscope. There was significant increment of motility by 13% and number of sperm by 40% compared to control, however there is no change in viability in both groups. Simultaneously, formulated herbals increased the number and motility (quality) of the sperm and potentially in solving infertility problem.

Index Terms—formulated herbals, mobility, viability of sperm

Cite:Ronny Lesmana, Hanna Goenawan, Aziiz M. Rosdianto, Akbar Haqi, Susianti, and Unang Supratman, "Combination Formulated Herbals (Pimpinella alpina, Eurycoma longifolia, and Curcuma xanthorrhiza) Increases Number and Improves the Quality of Sperm in Male Rat," International Journal of Pharma Medicine and Biological Sciences, Vol. 8, No. 3, pp. 106-109, July 2019. doi: 10.18178/ijpmbs.8.3.106-109

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