Abstract—We have previously reported the discovery of pyrazole derivative EH-1 displayed biological activity on inducing triple response in dark grown Arabidopsis seedlings. To improve our understanding of the action mechanism of EH-1, we conducted RNA-sequencing analysis of EH-1 treated Arabidopsis seedlings. Data present in this article for the first time demonstrated that EH-1 did not significantly induce ethylene responsive genes of ethylene receptors and/or ethylene signaling components in compared with none chemical treated control. However, we found that the expression levels of several genes were significantly increased by the treatment of EH-1 and ACC, the precursor of ethylene biosynthesis. This result indicates that the EH-1 induced the expression of some genes which are common to ethylene. We also found that there are several genes significantly induced by EH-1 but not induced by ACC. Our results suggest that the action mechanism of EH-1 shares with ethylene on inducing triple response in Arabidopsis seedlings but the primary site of action between EH-1 and ethylene are different.

Index Terms—ethylene, triple response, RNA Seq, plant growth regulators

Cite:Keimei Oh, Kenji Ueda, and Kojiro Hara, "Analysis the Action Mechanism of Pyrazole Derivatives EH-1 that Induces Triple Response in Arabidopsis Seedlings by Using RNA-Sequencing," International Journal of Pharma Medicine and Biological Sciences, Vol. 8, No. 2, pp. 45-48, April 2019. doi: 10.18178/ijpmbs.8.2.45-48