Abstract—The objective of this research was to evaluate potency of Eriocaulon cinereum R.Br to inhibit the HeLa cells. Extraction method was obtained by ultrasound-assisted extraction at 40 degrees up to 30 minutes with ethanol 96%. The extract was evaluated for total phenolic and flavonoid content and determine the proliferative on cervical cancer cell (HeLa) by MTT assay method. The result of total phenolic content is 18,983 mg/g of dry weight extract, expressed as gallic acid equivalents. The result of total flavonoid content is 63,518 mg/g, expressed as quercetin equivalents. Eriocaulon cinereum R. has potential as an anticancer with IC50 427,79 μg/ml on HeLa cells. This study has revealed the potential of Eriocaulon cinereum R.Br from Bangka Belitung Island for cervical cancer treatment. The study has shown that an in vitro exposure of HeLa cells to Eriocaulon cinereum R.Br extract, inhibit the growth HeLa cells.

Index Terms—Eriocaulon Cinereum R.Br, ultrasound-assisted extraction, MTT assay, HeLa cells, cervical cancer

Cite:Arde T. Nugraha, Viren Ramadhan, Herianto Pandapotan, and Fitra Romadhonsyah, "A Study of Proliferative Activity of Herbs Eriocaulon Cinereum R.Br on Cervical Cancer Cells (HeLa) with MTT Assay Method," International Journal of Pharma Medicine and Biological Sciences, Vol. 6, No. 2, pp. 73-76, April 2017.doi: 10.18178/ijpmbs.6.2.73-76