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Selection of Oleaginous Yeasts with Lipid Accumulation by the Measurement of Sudan Black B for Benefits of Biodiesel

Kusumawadee Thancharoen, Auttaporn Malasri, Walailak Leamsingkorn, and Patchareeporn Boonyalit
Rajabhat MahaSarakham University/Department of Biology, MahaSarakham, Thailand
Abstract—Microbial lipids possess significant potential which can be explored for biodiesel production. This study attempted to screened oleaginous yeasts and selected the strain that accumulated the largest quantity of lipid for lipid production from glucose. Among the 24 yeast strains isolated from canteen wastewater, 10 oleaginous yeasts were selected by Sudan Black B test. Sudan Black B staining revealed the presence of lipid inclusion bodies in the cells during cultivation under nitrogen-limiting conditions. Total oleaginous strains that accumulated quantities of lipid higher than 20% of their biomass when cultivated in glucose. It was found that accumulated the CTWY07 highest lipid content, up to 71.59% of its biomass (4.904 g/L lipid and
6.85 g/L biomass) , while CTWY17 grew the fastest by
shaking flask cultivation in glucose. The results show that
the isolated yeasts could be promising candidates for
biodiesel production.
 
Index Terms—biodiesel, lipid accumulation, oleaginous, screening, single cell oil, Sudan Black B

Cite:Kusumawadee Thancharoen, Auttaporn Malasri, Walailak Leamsingkorn, and Patchareeporn Boonyalit, "Selection of Oleaginous Yeasts with Lipid Accumulation by the Measurement of Sudan Black B for Benefits of Biodiesel," International Journal of Pharma Medicine and Biological Sciences, Vol. 6, No. 2, pp. 53-57, April 2017. doi: 10.18178/ijpmbs.6.2.53-57
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