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Undefined Organic Additives Stimulates in Vitro Seed Germination of Dendrobium Ovatum (Willd.) Kraenzl, a Medicinal Orchid

Thejaswini R and Narasimhan S
Department of Biotechnology, Manipal Institute of Technology, Manipal University, Manipal, India
Abstract—Dendrobium ovatum (Willd.) Kraenzl is an epiphytic orchid belonging to the family Orchidaceae. The aim of the current study is to analyze the efficacy of undefined additives such as coconut water, palm fruit water (Borassus flabellifer), tomato juice and pineapple juice on the germination and growth of in vitro cultured seeds of D. ovatum. The germination was analyzed using scanning electron microscopy (SEM). Among all the additives 30% coconut water (v/v) induced maximum rate of embryo germination . Tomato juice and pineapple juice (15% v/v) also exhibited better germination rate of orchid seeds under in vitro conditions. Protocorm like bodies were seen growing and further develops into young plant. SEM served as an early analytical tool for the response of seeds and revealed heterogeneity of seeds in terms of its response towards germination.

Index Terms—Coconut water, In vitro germination, Undefined additives

Cite: Thejaswini R and Narasimhan S, "Undefined Organic Additives Stimulates in Vitro Seed Germination of Dendrobium Ovatum (Willd.) Kraenzl, a Medicinal Orchid," International Journal of Pharma Medicine and Biological Sciences, Vol. 6, No. 1, pp. 29-31, January 2017. doi: 10.18178/ijpmbs.6.1.29-31
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