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Degree of Deacetylation of Chitosan Extracted from White Snapper (Lates sp.) Scales Waste

Noverita D. Takarina, Aldila A. Nasrul, and Alinda Nurmarina
Department of Biology, Universitas Indonesia, Depok, Indonesia
Abstract—Chitosan is natural biopolymer which can be obtained from natural resources even from wastes such fish scales. Indonesia is one of countries that having high fish production. White snapper (Lates sp.) is seawater fish that has an economical value since it is preferred by many consumers. New challenge to this condition is how to manage the waste from fish processing into useable materials. This study was aimed to extract chitosan from white snapper (Lates sp.) scales and to determine the degree of deacetylation of the chitosan based on the treatment differences in temperature, heating duration, and the NaOH concentration. Chitosan from white snapper scales were extracted through three stages, deprotenization, demineralization and deacetylation. Different treatment on deacetylation stages based on variation of NaOH concentration, temperature and duration of heating. Degree of deacetylation were measured using FTIR spectrophotometry based on infrared light absorbance. Result showed that chitosan produced were fulfilled minimum requirement for good quality of chitosan. Highest deacetylation degree was 84.05 %, resulted from treatment number 15 with NaOH concentration of 80%, temperature of 120oC and duration of heating for 4 hours. 

Index Terms—chitosan, degree of deacetylation, fish scale, white snapper, FTIR

Cite: Noverita D. Takarina, Aldila A. Nasrul, and Alinda Nurmarina, "Degree of Deacetylation of Chitosan Extracted from White Snapper (Lates sp.) Scales Waste," International Journal of Pharma Medicine and Biological Sciences, Vol. 6, No. 1, pp. 16-19, January 2017. doi: 10.18178/ijpmbs.6.1.16-19
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