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Cellular Thermal Measurement and Characteristic Analysis of Yeast Cells by Dielectrophoresis

Ryo Kido and Kozo Taguchi
Science & Engineering, Ritsumeikan University, Kusatsu, Japan
Abstract—In this paper we measured the cellular thermal changes by dielectrophoresis when we used yeast cell for the target cell. First, we conformed about heat damage evaluation of the yeast cells in the suspension. This is because to check the effectiveness of our proposed experimental systems and our electrodes. As a result, it was found that cells activity could be checked from shunt voltage changes. Second, we conformed about cellular thermal measurement using yeast cell. As the results, yeast cell was trapped between the electrodes by the DEP force and shunt voltage changes were measured as the impedance changes between the electrodes. Shunt voltage increased with the temperature rise, and decreased with the temperature drop. In addition, this phenomenon was confirmed only when the cells have been trapped. Also, when the number of trapped cell increased, voltage changes became larger.

Index Terms—dielectrophoresis, impedance measurement, yeast cell, protoplast, cellular thermal measurement

Cite:Ryo Kido and Kozo Taguchi, "Cellular Thermal Measurement and Characteristic Analysis of Yeast Cells by Dielectrophoresis," International Journal of Pharma Medicine and Biological Sciences, Vol. 4, No. 4, pp. 213-217, October 2015.

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