Abstract—Rice straw that has been treated with hydrochloric acid or sulfuric acid followed by heat treatment was used for the production of endo- and exoglucanase. Solid state fermentation was carried out with the following condition: inoculum size, 10%; moisture content, 50%; ammonium sulphate, 1%. After 6 days of fermentation, endo-glucanase and exo-glucanase were extracted; subsequently, enzyme assays were carried out. The highest exo-glucanase and endo-glucanase activity obtained were 8.16 ± 0.12 U/grice straw and 11.25 ± 0.14 U/grice straw, respectively using the acid/heat treated rice straw which was soaked in 15% of sulfuric acid for 2 hours. Conclusively, there was interaction between soaking time, concentration of acid and type of acid, and significant (p < 0.05) improvement in glucanase yield when compared to untreated rice straw.