Abstract—Effectiveness of structured teaching program on knowledge regarding prevention of urinary tract infection was conducted among 119 adolescent girls (experimental 56 and control 63) using quantitative pre test-post test control group design. Subjects were selected by one stage cluster sampling. Data were collected using structured questionnaire. Results: The study result showed statistically significant difference in gain in knowledge regarding prevention of urinary tract infection in the experimental group who had attended the structured teaching program (t (117) = 4.973, p< 0.001). Amount of water intake per day, voiding habits and hygienic practice during menstruation were the main contributing factors identified in the study population. Knowledge was significantly associated with the contributing factors of urinary tract infection like frequency of voiding during school hours ( χ ² ₍₁₎  = 12.930, p<0.05), voiding in unclean toilet ( χ ² ₍₁₎ =12.296, p<0.05), taking bath during menstruation ( χ ² ₍₁₎ = 5.761, p<0.05) and cleaning genitalia during menstruation ( χ ² ₍₁₎ = 4.145, p<0.05).