Abstract—Malaria is one of the major public health challenges and syndromic approach is unreliable because of non-specific and overlapping symptoms with other febrile diseases. Due to emerging drug resistance, accurate diagnosis of malaria is essential to start rational therapy for malaria. Various diagnostic techniques available include: Peripheral blood film, acridine orange staining, rapid diagnostic tests. Materials and Methods: This three years study was conducted in department of microbiology, Maharishi Markandeshwar Institute of Medical Research & Sciences (MMIMSR), Mullana, Ambala. The blood samples collected from 218 clinically suspected cases of malaria were subjected to Giemsa staining, acridine orange staining and Rapid Diagnostic Test (RDT) as per standard procedures. Results: In the present study, out of total 218 cases tested, 19.7%, 18.8% and 17.% were positive by Giemsa stain, acridine orange staining procedure and RDT, respectively. On further examination of positive cases; P. falciparum was detected in70%, 80% and 90% cases and P. vivax in 90%, 82.5%, and 75% cases by Giemsa staining, acridine orange and RDT, respectively. Conclusion: For P. vivax and for P.falciparum , Giemsa stain and RDT have better diagnostic accuracy, respectively.