Abstract—Introduction: Dengue is a common arthropod borne viral infection caused by any of the four serotypes of dengue virus. It can lead to life threatening complications such as dengue hemorrhagic fever. An increase in the number of cases of dengue has been noticed in India during recent years. Aims and Objectives: The present retrospective study was undertaken to know the trend of seroprevalence of dengue from 2007 to 2012 in a tertiary care hospital. Materials and Methods: A total of 2,405 blood samples from clinically suspected dengue patients were received in Microbiology laboratory during the study period of six years. Serum was separated from all blood samples and was further tested for the presence of IgM and IgG anti- dengue antibody by dengue IgM capture ELISA( MAC-ELISA, Pan Bio, Australia) and dengue Duo Cassette rapid strip test (Pan Bio diagnostics, Australia). Results: Out of 2405 serum samples, 720 (29.9%) were positive for IgM and IgG antibodies. Out of 720 positive samples, IgG antibodies were detected in 289/720 (40.1%), followed by IgM+ IgG antibodies 238 (33%),193/720 (26.8%) and IgM antibodies 193/720 (26.8%). Conclusion: In this study, we conclude that dengue cases are on an increase in Delhi, Haryana and adjoining region in the last five years (2007-2012). Therefore, health authorities and people of this region should make efforts to prevent further increase in dengue cases.