Abstract—Background: Mentat, a polyherbal psychotropic preparation, used for its memory enhancing property. Limited studies established its antianxiety property, so we evaluated antianxiety profile of mentat in animal models. Materials and Methods: Antianxiety activity of mentat was assessed in four groups of rats (n=6). The animals were treated orally (p.o.) with 300 and 600 mg/kg of mentat for seven days. On seventh day animals were subjected to Elevated Plus Maze (EPM) test, alprazolam (0.25 mg/kg), was a reference drug. Increase in time spent and number of entries in open arm was considered as an index of antianxiety activity. Results: Mentat exhibits dose dependent antianxiety activity in EPM. Mentat in dose of 300 mg/kg depicted significant antianxiety activity as compared to control group only (P\<0.001), while at dose of 600 mg/kg showed antianxiety activity, which was significant as compared to control and reference drug (P\<0.001) in EPM. Conclusion: Results of this study if substantiated by further experimental research suggests that mentat has antianxiety activity. Clinical trials document antianxiety profile of mentat but these trials had limitations like small sample size and single institutional setting. More research is required to develop novel plant products which are safe and have minimal adverse drug effect.