Abstract—Background: Non-Alcoholic Fatty Liver Disease (NAFLD) is an increasing global health concern, with an estimated prevalence of 20% to 30% in Western countries and 15% in Asian countries. Oxidative stress is considered a major contributor as the "second hit¡" in the pathogenesis of NAFLD. Objectives: We aimed to compare serum total antioxidant, MDA and Nitric Oxide levels in patients with NAFLD and normal individuals. Methods and Materials: Patients with NAFLD (n= 100) were compared to control subjects (n=100). Lipid peroxidation (Malondialdehyde) level was estimated using Thiobarbituric acid (TBA) method. Total antioxidant level was estimated using Phosphomolybdenum method. Nitric-oxide level was estimated using Griess-reagent method. Results are presented as mean + standard deviation value. Results: The mean serum level of total antioxidant in NAFLD individuals was found to be decreased when compared to normal individuals, whereas the levels of MDA and nitric oxide was found to be significantly increased compared normal individuals. Conclusion: This study may provide clinicians to draw different baselines for total antioxidant, Lipid peroxidation and Nitric oxide levels in patients with Non-Alcoholic Fatty Liver Diseases.