Abstract—This study on evaluation of Saline Wet Preparation, Staining and Cultural techniques of Trichomonas vaginalis among pregnant women in three selected hospitals covering rural and urban area in Oyo state. 150 samples of High Vagina Swab collected from both symptomatic and non-symptomatic were analyzed. Wet preparation, staining and culture were carried out. 38 (25.3%) of the women were found to harbor Trichomonas vaginalis . It was found that this parasite is present predominantly in female between the age of 29-31 years and are more common within the third trimester period. Also there were cases of multiple infection especially Candida spp. Results obtained in this study showed that the geographical distribution had no effect on the occurrence of Trichomoniasis as the difference was statistically not significant ( p = 0.6051). Results obtained from this study also showed that the best method of diagnosis is the cultural technique ( p = 0.001) among the three methods used.