Abstract—The antibacterial activity of petroleum ether, chloroform and ethanol extracts of the leaf and seed of Delonix regia and Achyranthus aspera were evaluated using agar well diffusion method against five bacterial strains namely, Bacillus subtilis, Escherichia coli, Enterobacter aerogenes, Micrococcus luteus, Pseudomonas aeruginosa. Chloramphenicol was used as standard.Among the three solvents analysed in leaf and seed, chloroform seed extract of D. regia and ethanol seed extract of A. aspera exhibited a high inhibitory zone against E. coli than other bacterial strains. Hence the phytoconstituents present may be used as antibiotic drugs.