Abstract—Objective: Is to investigate the prevalence of extended-spectrum 㰰〴㔾 -lactamases among Escherichia coli isolates in a tertiary care hospital in Osogbo. South western Nigeria. Materials and Methods: A total of 255 clinical isolates of E. coli ( n = 255) were recovered from various clinical samples over a period of twelve months from October 2010 to September 2011 and there antimicrobial susceptibility testing was determined to commonly used antibiotics using the modified Kirby-Bauer¡¯s disc diffusion method. ESBL detection was done by the screening method of double disc synergy test as recommended by the Clinical Laboratory Standards Institute (CLSI) Results: Out of 255 isolates of E. coli screened for ESBL production, 105 were found to be potential ESBL producers. Of these, 14 isolates were confirmed to be ESBL producers. Thus the prevalence of ESBL-producing isolates of E. coli was found to be 5.5% (14 out of 255). We also report a high percentage of resistance to Augumentin (84.3.%) while Imipenem has the highest percentage of sensitivity, 249 (97.5%). Conclusion: In conclusion ESBLs were present in the study location and were resistant to most antibiotics applied. This has a significant implication for patients. We therefore suggest that routine and confirmatory tests should be included in routine diagnostic laboratory work.

Index Terms—Escherichia coli , extended-spectrum ß-lactamase, Third-generation cephalosporins

Cite: Olowe O A, Ayilara O A, Oladipo G O, Makanjuola O A, and Olaitan J O, "Multidrug Resistance Escherichia coli Carrying Extended-Spectrum β-Lactamases Enzymes in a Tertiary Care Hospital in Osogbo, South Western Nigeria," International Journal of Pharma Medicine and Biological Sciences, Vol. 1, No. 2, pp. 143-149, October 2012.