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Prevalence of Extended Spectrum Beta-Lactamases (ESBLs) Carrying Genes in Klebsiella Spp from Clinical Samples at Ile-Ife, South Western Nigeria

Olowe O A1, Oladipo G O2, Makanjuola O A1, and Olaitan J O3
1 Department of Medical Microbiology and Parasitology, College of Health Sciences, Ladoke Akintola University of Technology (LAUTECH) Oshogbo, P.M.B 4400, Osun state, Nigeria.
2 Department of Medical Microbiology and Parasitology, ObafemiAwolowo University Teaching Hospitals Complex (OAUTHC) Ile- Ife, PMB 5538, Ile-Ife, Osun State, Nigeria.
3 Department of Microbiology, Osun State University, Osogbo, Nigeria.
Abstract—Extended Spectrum Beta Lactamases (ESBLs) that mediate resistance to 3 rd generation cephalosporins were investigated. The aim of this study was to determine the prevalence ESBLs genes among Klebsiella pneumoniae isolated from different clinical specimens of patients admitted in Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC), Ile ¨CIfe, Osun State, from October 2010 to April 2011. All isolates were screened and confirmed for extended spectrum beta- lactamase (ESBL) production by Kirby-Bauer disk diffusion method on Mueller Hinton agar using CLSI guidelines. ESBL production was phenotypically determined using double disc synergy test and subjected to PCR for genotyping. Two hundred isolates were recovered, 66(33%) K. pneumoniae , 12(6%) K. oxytoca , 39(19.5%) P. aeruginosa , 33(16.5%) Proteus species , 15(7.5%)) Escherichia coli, and 35(17.5%) S. aureus . ESBL producing K. pneumonia were most frequently isolates from sputum 63(32%) followed by blood 43(21.5%), wound 39(19%), urine 32(16%), HVS 12(6%) and CSF with least incidence of 11(5.5%). By disk diffusion, the strains were resistant to almost antibiotics while Trimethoprim-Sulphamethoxazole showed highest ressistance of (96.3%), while sensitivity to imipenem was 100%. The prevalence of 32% was recorded for bla TEM, while bla SHV and bla CTX-M, were 32% and 36% respectively.

Index Terms—Extended Spectrum Beta-Lactamase (ESBL), Klebsiella species, Prevalence, Antimicrobial resistance, Double-disc method.

Cite: Olowe O A, Oladipo G O, Makanjuola O A, and Olaitan J O, "Prevalence of Extended Spectrum Beta-Lactamases (ESBLs) Carrying Genes in Klebsiella Spp from Clinical Samples at Ile-Ife, South Western Nigeria," International Journal of Pharma Medicine and Biological Sciences, Vol. 1, No. 2, pp. 129-138, October 2012.
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