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Longitudinal Study on the Effect of Restraint Stress during Preweaning Period on Postnatal Growth of Body and Constituent Parts in Albino Mice

Prakash Billakanti Babu, Ramachandra Bhat K, and Muddanna S Rao
Anatomy Department, Kasturba Medical College, Manipal University, Manipal, Karnataka-576104, India.
Abstract—Stress is the phenomenal; any stimulus which displaces the state of normal physiological function can cause stress and has its effect in various forms. Somatic growth and development are also affected by stress affecting growth releasing hormone. This study was conducted to observe growth pattern in normal and stressed condition in growing mice. Experiments were conducted to investigate the effect of restraint stress applied at different times of the light-dark cycle on growth rates (body and tail length and body weight of rats). BALB/ C strain of Albino rats were restrained for 6 hours daily from birth to end of three weeks (21 days) 6 hourly from birth till postnatal day 21 (21 days stress) and from postnatal day 16 to 21 day (5 day stress) in a restraining device, which consisted of a wooden platform to which a wire-mesh was attached. Body weight (BW), tail length (TL) and body length (BL) was recorded at the beginning and at the end of stress both in control and experimental groups. Recorded parameters were subjected to statistical analysis between the groups. Results of the study revealed that stress affects body weight significantly and longitudinal growth of body and tail lengths of albino mice.

Index Terms—Albino mice, Restraint stress, Body measurements

Cite: Prakash Billakanti Babu, Ramachandra Bhat K, and Muddanna S Rao, "Longitudinal Study on the Effect of Restraint Stress during Preweaning Period on Postnatal Growth of Body and Constituent Parts in Albino Mice," International Journal of Pharma Medicine and Biological Sciences, Vol. 1, No. 2, pp. 80-90, October 2012.
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